Procedure for handling of cultures & contaminated material

 STANDARD OPERATING PROCEDURE

Title :Procedure for handling of cultures & contaminated material

Department:Quality Control-Microbiology

• OBJECTIVE

• To lay down a procedure for handling of cultures & contaminated material.

• SCOPE

• This SOP is applicable for handling of cultures & contaminated material in microbiology laboratory .

• RESPONSIBILITY

• Officer / Executive- QC M – responsible for Implementation of SOP.

• ACCOUNTABILITY

• Manager – Quality Control

• PROCEDURE

• Used 2.5% Savlon /filtered 70% Isopropyl alcohol Solution to disinfect the spillage.
• Wear hand gloves and spray /pour the disinfectant solution on the spilled medium/spilled culture.
• If glassware is broken, pick up the broken glass pieces. Soak the broken glass pieces to the container meant for collection of broken glassware.
• Swab the area of spillage to remove the media traces of medium/ microbial culture using a sterile non shedding duster soaked in disinfectant solution.
• Wipe clean the area of spillage with a sterile non shedding duster soaked in disinfectant solution.
• In case of aerosol has been created during spillage, fumigate the area on same time.
•  In case of spillage of medium/ culture on LAF bench, clean the spillage with sterile non-shedding duster soaked in disinfectant solution. Switch on the UV light for one hour before start any activity in LAF unit.

•         ABBREVIATION

SOP      –  Standard operating procedure

LAF      –  Laminar Air Flow

UV        – Ultra Violet

• FORMAT-    Nil.

• DISTRIBUTION LIST