Procedure for handling of cultures & contaminated material

 STANDARD OPERATING PROCEDURE

Title :Procedure for handling of cultures & contaminated material

Department:Quality Control-Microbiology
  • OBJECTIVE
  • To lay down a procedure for handling of cultures & contaminated material.
  • SCOPE
  • This SOP is applicable for handling of cultures & contaminated material in microbiology laboratory .
  • RESPONSIBILITY
  • Officer / Executive- QC M – responsible for Implementation of SOP.
  • ACCOUNTABILITY
  • Manager – Quality Control
  • PROCEDURE
  • Used 2.5% Savlon /filtered 70% Isopropyl alcohol Solution to disinfect the spillage.
  • Wear hand gloves and spray /pour the disinfectant solution on the spilled medium/spilled culture.
  • If glassware is broken, pick up the broken glass pieces. Soak the broken glass pieces to the container meant for collection of broken glassware.
  • Swab the area of spillage to remove the media traces of medium/ microbial culture using a sterile non shedding duster soaked in disinfectant solution.
  • Wipe clean the area of spillage with a sterile non shedding duster soaked in disinfectant solution.
  • In case of aerosol has been created during spillage, fumigate the area on same time.
  •  In case of spillage of medium/ culture on LAF bench, clean the spillage with sterile non-shedding duster soaked in disinfectant solution. Switch on the UV light for one hour before start any activity in LAF unit.

 

  •         ABBREVIATION

SOP      –  Standard operating procedure

LAF      –  Laminar Air Flow

UV        – Ultra Violet

 

  • FORMAT-    Nil.

 

  • DISTRIBUTION LIST
Sr. No. Copy No. Department
    Quality Assurance
    Quality Control-Microbiology
    VP – Plant & Operations

 

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