Procedure for handling of Microbiology Area

•     OBJECTIVE  
  • To lay down a procedure for handling of microbiology area.
• SCOPE
  • This SOP is applicable for handling of microbiology area.
• RESPONSIBILITY
  • Officer / Executive-QCM – responsible for Implementation of SOP.
• ACCOUNTABILITY
  • Manager – Quality Control
  • Manager – Quality Assurance
• PROCEDURE
• Cleaning of Microbiology Area:
  • Put any leftover waste on floor to waste bin.
  • All the waste bins in microbiology lab should be cleaned on daily basis.
  • Clean the area with disinfectant solution.
  • Weekly clean the doors, walls, glasses and monthly clean the ceiling with disinfectant solution like dettol, savlon,  70L% IPA.
•  Handling in Microbiology lab and MLT Room:

• • Remove the factory slippers outside microbiology lab.
  • Cap, Masks, Apron should be used in microbiology lab.
  • Put wastes after analysis in waste bin.
  • Always kept used glassware’s in tray containing 70% IPA.
  • Follow aseptic handling during testing to avoid external contamination.
  • Keep the LAF bench clean after use with 70% IPA.
  • Get the area clean immediately after use.
•     Sterility Room:

• •  Transfer sterilized test units & other articles through pass box to sterile area.
  • Remove factory clothes in air lock -2 & clean the hands with IPA 70%.
  • Use sterilized dress kept in air lock-2 prior to entering sterility test room.
  • Open the door of sterility room using elbows & enter in sterile area.
  • Switch on the LAF & allow running 30 minutes prior to testing & continued.
  • Transfer the articles from pass box to LAF bench.
  • Conduct the sterility test as per SOP No.
  • Put wastes after analysis in waste bin.
  • Used glass wares shall be kept in tray containing IPA 70%.
  • Follow aseptic handling during testing to avoid external contamination.
  • Keep the LAF bench clean after use.
  • Get the area clean immediately after use.
•     ABBREVIATION
  •    SOP      –  Standard operating procedure
  •    IPA       –  Iso Propyl Alcohol
  •   LAF        – laminar Air Flow
  •    MLT       – Microbial Limit Test
  •    UV         – Ultra Violet

•     FORMAT

Nil.

•   DISTRIBUTION LIST