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MFR of Amikacin Sulphate Injection

Posted on October 24, 2020December 22, 2023 By ABHA

MFR of Amikacin Sulphate Injection

PURPOSE: This Master Formula Record (MFR) is written to describe the formulae, manufacturing procedure, specifications, packing details of dosage form.

SCOPE: This MFR is performed and is applied during the manufacturing of dosage form.

Also Read:- MFR of Chloramphenicol EYE/EAR Drops

RESPONSIBILITY / ACCOUNTABILITY: It is the responsibility of  Manufacturing Chemist to follow and adhere to this MFR. The Production Manager, QC/QA Manager are accountable for the strict adherence to the master formula.

COPY ISSUED TO:

  1. Master Copy : Manager Quality Assurance
  2. Copy No. 1 : Production Manager
  3. Copy No. 2 : Manager Quality Control
  4. Copy No.   3 :   Small Volume Parenteral Section

PRODUCT NAME: MFR of Amikacin Sulphate InjectionBATCH SIZE: 100 LTRS.
PRODUCT REFERENCE CODE: UNIT SIZE:  2 ml/white/Vial
GENERIC NAME: Amikacin Sulphate Inj.                       PACK SIZE:  30 x 50 x 2ML
DOSAGE FORM: Parenteral dosage form STRENGTH: 250 mg/ml
DEPARTMENT:  STERILE PREPRATIONEXPIRY DATE: AFTER 24 MONTHS FROM THE DATE OF MANUFACTURING

Each 2ml contains: –

Amikacin SulphateI.P
Equivalent to Amikacin500 mg
Water for injectionI.Pqs

EQUIPMENTS TO BE USED: 

SR. NO.NAME OF EQUIPMENTASSEMBLING

AS PER SOP NO.

CLEANING

AS PER SOP NO.

1Auto Rotary Vial Washing Machine
2Butyl Bung Washing Machine
3Dry Heat Sterilizer
4Auto Clave with SS Transfer trolley
5Multi Column Distillation Still
6Distill Water Storage Tank
7SS Batch Mixing Tank
8SS Pressure Vessel – 100 Ltr.
9SS Membrane Filtration Assembly (293 mm)
10SS Filling Vessel  – 100 Ltr
11Auto Liquid Vial Filling, Stoppering & Sealing Machine
12Vial Visual Inspection Table
13Auto Vial Labelling Machine

Equipments to  be sterilized :- 

SR NONAME OF EQUIPMENTS STERLIZATION AS PER SOP NO.
1S.S Membrane vessel- 293 MM
2S.S Filling vessel
3S.S Filling syringes
4Sterile dress of operator
5Pre Nitrogen Filling needles& POST- nitrogen filling needles
6Silicon tubes

RAW MATERIALS: 

S.N.INGREDIENTS STDTheoretical Quantity Req.Overages %Total Quantity Used
1.AMIKACIN SULPHATEI.P.37.8005.0039.690 KGS
2.EDTAI.P.0.040–0.040 KGS
3.METHYL PARABEN SODIUMI.P.0.080–0.080 KGS
4.PROPYL PARABEN SODIUMI.P.0.020–0.020 KGS
5.S.M.B.SI.P.0.500–0.500 KGS
6.TRI – SODIUM CITRATE (SD)I.P.2.500–2.500 KGS

MANUFACTURING PROCESS:

Preparation of Solution:

Take in SS Batch Mixing Tank 60 Lt. of freshly prepared hot distilled water.

Dissolve Methyl Paraben & Propyl Paraben to Batch Mixing Tank Stir it properly till mix.

Dissolve EDTA SMBS and stir properly.

Add Tri sodium citrate and stir it properly.

Dissolve Amikacin Sulphate.

Adjust the pH to 4.5  (pH range: 3.5 to 5.5)

Make up the volume to 100.00 liter

Final pH = 4.5

Shift the solution from batch mixing tank to pressure vessel and do membrane

Filtration by using the appropriate membrane.

Washing

Washing of vials

Vial washing is done with auto rotary vial washing M/c as per sop.

Washing of Butyl Bungs

Butyl Bungs washing is done with Butyl Bungs Washing as per sop.

Sterilization

Dry Heat Sterilization (Vials)

Dry Heat Sterilization is done with the help of Dry heat Sterilizer at the following conditions as per sop.

1Temperature250°C
2Holding Time1 hour

Moist Heat Sterilization (Butyl Bungs)

Moist heat sterilization for butyl bungs is done with the help of Autoclave with SS Transfer Trolley at following conditions as per sop

APressure15 lbs
BTemperature121° C
CHolding Time30 Minutes

Filtration

Filtration is done with the membrane assembly by using the appropriate membrane & pre filter and post filter

APre Filter1.5  µ
BMembrane Filter0.2  µ

Aseptic Filing

After the process of washing, sterilization filtration filing of Aseptic filing is carried out in class 100 area under the laminar flow. The machine adjustment is carried out for the appropriate vials as per SOP.

AFilling M/C Adjustment
BButyl Bung Station Adjustment
CSealing Station Adjustment

Volume variation is checked intermittently, volume prescribed 2.ml and volume filled 2.0 ml, percentage variation 2%.

Visual Inspection

Visual inspection is carried out for filled vials by using vial visual inspection table M/c as per SOP.

Labeling

Labeling of vials is done with the help of auto vials labeling machine as per sop

Packing

1Open PackingYes/noYesPack SizeNA
2Blister PackingYes/noNoPack Size30 x 50 x 2ml

Yield

1Percentage Yield–100 ± 2%
2Theoretical Yield–50000 x 2ml
3Expected Practical Yield–50000 x 2ml ± 2%

Packing Details:

Label the filled vials &Blister the labeled vials

Pack such 10 labeled blister pack each of 5vial in each outer carton

Seal outer carton with cello tape

Pack the 30 outer cartons in specified corrugated box to give pack size of 30 x 10 x 5 x 2ml.

Seal the corrugated box with adhesive tape and label it properly by affixing the specified label

IN-PROCESS CONTROLS:-

THE following in process controls should be maintained during  the processing

Check raw  material used for manufacturing purpose are all approved materials and have ‘released’ Labels fixed on it.

All weighed Raw materials should be counter-checked by  Manufacturing Chemist. If any discrepancy is noticed, it should be immediately brought to the notice of Production Manager and QC/QA Manager.

Physical characteristics of Raw material like colour, odour and consistency are checked before compounding.

Final volume should be made as per Standard Operating Procedure.

pH of the bulk should be checked and it should be with in specified limits.

Bulk sample should be sent for analysis to Q.C. Department before starting the filling and sealing stage.

Intermittently filled volume should be checked at 30 minutes interval by the  Manufacturing Chemist and record for the same should be kept in Batch Manufacturing Record.

The net volume should be checked for all the filling nozzles and in no case net volume should be less than the volume claimed on the label. (Limit for volume variation: volume claimed + 2%.

Visual inspection of filled and sealed vials should be done as per SOP  and the record of the same should be kept in B.M.R.

The labels and cartons should be checked thoroughly for proper batch coding.

Intimation should be sent to Q.C. Department for finished product sampling and testing.

After the completion of labeling and packaging the coded labels and cartons should be accounted for and rejected printed material should be destroyed in the presence of QC/QA  Manager. Maintain the destruction of the same in the B.M.R.

It will be ensure that filling or packing equipment has been properly cleaned.

Filling or packaging of next product should not commence until the the ‘Line Clearance’ has been given by the Q.A. Analyst.

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